The present study was designed to determine the phyto components from the chloroformic extract of a poly herbal formulation ACTP were studied. The analysis of natural products involves isolation in a pure form of plant constituents and investigation of their structure, formation, efficacy and purpose in the organism. Secondary metabolites appear to function primarily in defense against predators and pathogens and in providing reproductive advantage as intraspecific and interspecific attractant. The present study aims at quantifying the phyto constituents by chromatographic methods. Therefore, it is of interest to investigate the GC-MS analysis of the chloroformic extract of the herbal formulation ACTP were analysed Our result envisage that the presence of 21 phyto constituents of a poly herbal formulation of ACTP.