In RP-HPLC method, the conditions were optimized to obtain an adequate separation of eluted compounds. Initially, various mobile phase compositions were tried, to separate title ingredients. Mobile phase and flow rate selection was based on peak parameters (height, tailing, theoretical plates, capacity or symmetry factor), run time and resolution. The mobile phase containing mixture of 0.1% OPA (pH-3): Acetonotrile (30:70v/v) with a flow rate of 1.5 ml/min is quite robust. The optimum wavelength for detection was 260 nm at which better detector response for both the drugs was obtained. The retention time of Eliglustat was found to be 2.144 min. To ascertain its effectiveness, system suitability tests were carried out on freshly prepared stock solutions. The calibration was linear in concentration range of 84 to 420 ?g/ ml, with regression 0.9979. The low values of % R.S.D indicate the method is precise and accurate. The mean recoveries were found above 100.53 % for the drug. The Method was checked by doing validation according to the ICH guidelines. Hence the method is validated and it is used for the routine analysis of Eliglustat in its pharmaceutical dosage form by RP-HPLC.
