Introduction: The long term interaction of mobile phone radio-frequency radiation (RFR) on general health, especially on male fertility and repercussion of above is becoming a major concern for current researchers for obvious reasons. Objective of the current study was aimed for evaluating the effect of long term exposure of RFR from mobile phones on blood pressure, hematological parameter, testosterone level, sperm count and their morphology. To perform the current study the RFR generator was used and a uniform radio frequency radiation with frequency range of 900 MHz and 1800 MHz were exposed to the rats placed individually in hexagonal plastic cage which is divided into six compartments. After the experimental period, blood pressure, hematological parameter, testosterone level, sperm count and their morphology were studied .Total sperm count, their morphology, total platelet count were significantly affected at both RFR. BP, neutrophil, lymphocyte percentage were significantly affected at 900 MHz however no significant changes were observed in hemoglobin content, total RBC and WBC count, eosinophil percentage and serum testosterone level.Hence it can be concluded that a long term (12week) mobile phone RFR exposure significantly affected hematological parameter, and had perilous effect on male fertility in rats.