A new method was developed for simultaneous estimation of Teneligliptin and Metformin by RP-HPLC. The chromatographic conditions were successfully carried out on Inertsil ODS column C8 (4.6x250mm) of 5?m particle size, flow rate was 1.0ml/min, using mobile phase composed of 50% buffer: 50% Acetonitrile, detection wavelength used as 240 nm. The instruments and materials used were WATERS, software: Empower, 2695 separation module.2487 UV detector, UV/VIS spectrophotometer-LABINDIA UV 3000+. The retention time was found to be for Metformin 3.608 min and for Teneligliptin 5.148 min. The assay of Metformin and Teneligliptin was performed with tablets and the % assay was found to be 99.97 and 100.64 which shows that the method is useful for routine analysis. The linearity of Metformin and Teneligliptin was found to be linear with a correlation coefficient of 0.999 and 0.999, which shows that the method is capable of producing good sensitivity.The acceptance criteria of precision is RSD should be not more than 2.0% and the method show precision 0.4 and 0.8 for Metformin and Teneligliptin which shows that the method is precise.The acceptance criteria of intermediate precision is RSD should be not more than 2.0% and the method show precision 0.1 and 0.7 for Metformin and Teneligliptin which shows that the method is repeatable when performed in different days also. The accuracy limit is the percentage recovery should be in the range of 97.0% - 103.0%. The total recovery was found to be 99.86% and 99.96% for Metformin and Teneligliptin. The validation of developed method shows that the accuracy is well within the limit, which shows that the method is capable of showing good accuracy and reproducibility. The acceptance criteria for LOD and LOQ is 3 and 10. The LOD and LOQ for Metformin was found to be 3.00 and 9.98 and LOD and LOQ for Teneligliptin was found to be 3.02 and 10.00. The linearity of Teneligliptin and Metformin was found to be linear with a correlation coefficient of (R2) 0.998 and 0.999 respectively. The robustness limit for mobile phase variation and flow rate variation are well within the limit, which shows that the method is having good system suitability and precision under given set of conditions. The analytical method was validated according to ICH Guidelines.