The present study aims to investigate and characterize the chemical composition of the chloroform extract of a novel poly herbal formulation was studied. The air dried plant materials were powdered and further subjected to gas chromatography and mass spectrometry. Qualitative determination of the different Pharmacologically and biologically active compounds from chloroform extract of a novel poly herbal formulation using gas chromatography and mass spectrometry revealed different types of high and low molecular weight chemical entities with varying quantities present in the extract. These chemical compounds are considered biologically and pharmacologically important. Furthermore, the chloroform extract of a novel poly herbal formulation possess unique physicochemical characteristics which may be attributed to the compounds naturally present in significant quantities in the herbal formulation. The chloroform extract of a novel poly herbal formulation possess 13 major bioactive compounds that were identified and characterized spectroscopically. Hence, identification of different bio pharmacologically active compounds may warrants further biological and pharmacological studies.
