Abstract
Natural products from medicinal plants, either as pure compounds or as standardized extracts, help to overcome unmet medical needs. Phytoconstituents of many medicinal plants are unknown. One of such plant is Merremia emarginata (Burm.f.) Hallier.f. this plant has been reported for many biological activities but phytoconstituents are still unknown. Due to increasing demand for herbal drugs thereby seeking of therapeutic drugs from medicinal plants is necessary but these plants vary in content from regions and seasons hence demand for screening of herbal formulation and standardization of process is required. Process standardization will develop a quality product and hence safety and efficacy of herbal drugs can be improved. Herbal preparations for medicinal usage contain various types of bioactive compounds. Merremia emarginata (Burm.f) Hallier f.commonly known as Akhuparni is a medicinal Plant belonging to the family Convolvulacea. The plant has been used in many herbal formulations, but phytoconstituents of the plants are unknown except the broad categories. The present study reports the isolation of phytoconstituents by means of Supercritical fluid extraction and separation of isolates by means of HPLC and characterisation of purified isolates by FTIR, HRMS & NMR. JASCO SFE system of 2000series was used for extraction of compounds from plants, ethanol was used as modifier for the extraction and back pressure of system was maintained at 15MPa for 30mins. The extract was further isolated and purification was detected with help of JASCO HPLC-PDA detector. The Purified compounds were identified as Quercitin, Kaempferol & Naringin using characterisation techniques mentioned above.
