Abstract

A novel, simple and economic reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for the simultaneous estimation of Levosulpiride and Rabeprazole sodium in bulk and tablet dosage form with greater precision and accuracy. Separation was achieved on C18 column (250X4.6mm i.d.,5μm) in isocratic mode with mobile phase consisting of acetonitrile, methanol and potassium dihydrogen phosphate (pH was adjusted to 7.4 by using Triethylamine) in the ratio of 50:25:25(v/v/v) at a flow rate 1 mL/min. The detection was carried out at 289nm for both drugs. The retention times of Levosulpiride and Rabeprazole sodium were found to be 2.76 and 4.20 min respectively. The method was validated as per ICH guidelines. Validation studies demonstrated that the proposed RP-HPLC method is simple, specific, rapid, reliable and reproducible. The high recovery and low relative standard deviation confirm the suitability of the proposed method for the simultaneous estimation of these drugs in bulk and tablet dosage.