Abstract
The objective of the study is to assess antibacterial activities of essential oil and its mixture of two Artemesia species against four bacterial strains isolated from urine (Escherichia coli, Pseudomonas aeruginosa, Proteus spp. and Klebsiella spp.). The essential oils percentages of the aerial parts of Artemesia herba alba and Artemesia monosperma were 0.83 % and 0.22 %, respectively as clear yellowish liquid oil. Mixing essential oil of both plants with ratio (1:2 v: v), respectively, gave highest antibacterial activity, then the mixture were investigated using GC –MS, beside investigation the essential oil of each plant alone. The obtained results showed that, the major constituents of the essential oil of Artemesia herba alba were Geraniol (28.27 %), Artemesia alcohol (24.92 %), Yomogi alcohol (13.05 %) and αˈ-Ionol (11.66 %), which resembled monoterpene alcohol compounds. Meanwhile the major constituents of the essential oil of Artemesia monosperma were Naphthalene 2-ethenyl (Aromatic hydrocarbon) (35.20 %), Imidazolidine (Heterocyclic compound) (19.27 %), and 2αˈ-pinene (Monoterpenes) (10.89 %). On other-hand the obtained major components of the mixture (1: 2, v: v) were Naphthalene 2-ethenyl (31.78 %), Imidazolidine (14.84%) and 2αˈ-pinene (12.93 %). Meanwhile the mixture of Artemesia herba alba and Artemesia monosperma with ratio (1: 2 v: v, respectively) showed highest antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Proteus spp. and Klebsiella spp. isolated from urine. The present study scientifically proved that the mixture of essential oil of Artemesia herba alba and Artemesia monosperma is antimicrobial agent that can be used to treat urinary tract infectious diseases.
