Abstract

The present work was aimed to study effect of amount of polymer, surfactant concentration and volume of organic phase on Particle size and %entrapment efficiency. The response surface methodology based on a three-level, three-variable Box - Behnken design was used to evaluate the interactive effects on dependent variables. Vasopressin loaded nanoparticles were prepared by double emulsion solvent evaporation method, using mixture of PLGA and PCL, as biodegradable carrier. The study showed that Particle size and Entrapment efficiency both are greatly influenced by amount of polymer as independent factor. Surfactant concentration also contributes to reduced particle size at significant level. Though volume of organic phase showed negative attribute towards entrapment efficiency. The formulation was optimized by selecting constraints of minimum particle size and maximum entrapment efficiency. Optimized batch of nanoparticles was characterized for various parameters. SEM confirms spherical shape of nanoparticles; showed initial burst release with prolonged drug release, followed Higuchi model indicated controlled delivery of drug that can be associated for reducing the frequency of drug administration for better patient compliance.