Abstract
Plants are important for their medicinal value because they contain numerous compounds with therapeutic value. Is widely used in traditional system of medicine. Citrus limonum is screened for antimicrobial activity and antihelminthic. The antifungal and antibacterial activity of leaf extract was determined by well method. The methanolic leaf extract had showed significant antibacterial activity at 100 μg/ml on E.coli when compared to that of standard drug streptomycin. In case of antifungal activity the zone of inhibition is not seen at any concentrations of 25 μg/ml, 50μg/ml, 75μg/ml and100μg/ml of the leaf extract. The present activity also includes anthelminthic activity performed on methanolic extracts concentrations at 10 mg/ml, 20 mg/ml & 50 mg/ml of leaf extract Citrus limonum by using pheretima posthuma as test worms. The time paralysis and time of death were studied and the activity was compared with piperzine citrate as reference standard. Dose dependent activity was observed in the extracts. This study provides an explicit perception about the presence of portion (leaf), to which one has more number of phytochemicals. The methanolic extract of the leaf of Citrus limonum revealed the presence of carbohydrates, proteins, amino acids, steroids, volatile oils, tannins are present.
