Abstract
Chick Embryo Chorio Allantoic Membrane [CAM] is an extra embryonic membrane used mainly for gas exchange and transport of calcium from the egg shells to the embryo during its development. It is used to study new blood vessel formation and its inhibition in response to tissues, cells or soluble factors such as antibodies. CAM is broadly used in oncology, biology, pharmacy and tissue regeneration research. There are 46 morphologically distinct stages of chick development beginning with a freshly laid egg and ending with a fully developed and hatched chick. These stages help provide consistency and coordination between the various areas that use the chick embryo model. It is a novel technique to facilitate the discovery and development of newer drugs, circumventing the use of animals. Due to its ease of availability, extensive vascularisation, and immuno deficient environment, it is used to study morpho functional aspects of angiogenesis process in vivo. Progress in imaging and chick culture technologies is advancing real-time visualization of dynamic biological events. Cell line studies have attained utmost importance due to advanced technologies and are used as an alternative to animal experiments. Jurkat cells derived from T Lymphocyte have been developed to provide a link between the Immunomodulatory and anti angiogenic aspect of the drug. The present study was carried out to evaluate the anti-angiogenetic activity of crude extract of Anogeissus latifolia using chick embryo chorioallantoic membrane and cell line models. Direct drug injection and disc impregnation methods were followed for chick embryo. Sulphorhodamine B assay was followed for cell line study. Reduction in the blood vessel branching was observed in chorio allantoic membrane and reduction in cell number was observed for cell line studies indicating that the drug possess anti angiogenic activity.
