Abstract

Highly sensitive, simple and economic spectrophotometric and fluorimetric methods were developed for the determination of posaconazole (POS) in pharmaceutical preparations and spiked human plasma through solvent solvent extraction technique. The proposed methods are based on measuring the absorbance of the drug in methanol at 262 nm and the native fluorescence intensity in isopropanol at 378 nm after excitation at 262 nm. The absorbance –concentration plot and fluorescence intensity-concentration plot were rectilinear over the range 0.612-17.500 μg/ml and 22-550 ng/ml with a lower detection limit of 0.202 μg/ml for the spectrophotometric and 7 ng/ml for the fluorimetric analysis and the mean percent recovery were found to be 100.192 and 100.002 for spectrophotometric and fluorimetric techniques respectively. Both methods were validated according to ICH guidelines and showed good accuracy and precision with no significant difference compared with the reported HPLC method. It can be concluded that these methods can be used for determination of POS in dosage form and spiked human plasma.