Abstract
The construction and performance characteristics of novel trimetazidine (TMZ) solid contact potentiometric sensors were introduced. The cited electrodes are based on alpha cyclodextrin (α-CD) as supramolecular receptors which show selectivity towards TMZ. Electrode matrix compositions were optimized on the basis of effects of type and concentration of the sensing ionophore as well as influence of ionic sites and plasticizers. Sensor incorporated with α-CD, potassium tetrakis (4-chlororophenyl) borate (KTClPB) and 2-fluorophenyl 2-nitrophenyl ether (f-PNPE) as membrane plasticizer showed the highest electroanalytical performances. The fabricated sensors work satisfactorily in the concentration range from 1.0×10-7 to 10-2mol L-1 with cationic Nernstian slope value 34.42± 0.57 mV decade-1 corresponding to trimetazidine dihydrochloride. Fast response times of about 3 s and adequate shelf-life (12 weeks) were achieved. Application of cyclodextrin as a recognition elements improved the selectivity of the fabricated sensors compared with tradition ion-association compounds. The fabricated sensors showed satisfactory sensitivity and selectivity towards trimetazidine and can be introduced as suitable tool for the routine quality control analysis of TMZ in its pharmaceutical under batch and FIA potentiometric measurements. FIA allows the analysis of 70 samples h-1 and offer the advantage of simplicity, accuracy and automation feasibility. Comparison of the obtained results with those provided by reference official method revealed adequate accuracy for control assay.
