ABSTRACT
This paper describes production and characterization of therapeutic and industrially potent enzymes from Streptomyces albogriseolus (M10A99) as well as antagonistic activity of Streptomyces brasiliensis (M10A85). The actinobacterial strains were isolated from magnesite mine soil, Salem, Tamil Nadu, India. The enzymes (L-asparaginase, chitinase and pectinase) were produced by submerged fermentation at 1 % of L-asparagine, chitin and pectin used as substrate respectively. The maximum activity of the enzyme was observed at pH 7, temperature 40° C and 500 µg of substrate concentration. The presence of metal ions EDTA and Zn2+ were inhibited the enzyme activities but Cu2+, Fe2+ and Mg2+ were increased the enzyme activities. The enzyme was partially purified by using (85 %) ammonium sulphate precipitation, dialysis and DEAE - cellulose chromatography. The partially purified enzyme showed the activity, 1534.20, 1076.80 and 978.90 IU/ml, respectively. The molecular weight of the enzymes were approximately found to be about 98, 32 and 35 kDa. The bioactive compound from potential strain was produced by agar plate solvent extraction method using solid state fermentation. The potential compound was partially purified by thin layer chromatography and bioautography tested against clinically resistant pathogens. The bioactive compound was characterized by UV- Vis spectroscopy and fourier transform infrared red (FT-IR) spectroscopy. Finally, the potential strains were characterized and identified by using Nonomura key’s and actinobase database.
