Abstract
The present study was aimed to reveal the phenolic and steroid profile of two varieties of Blechnum orientale L. viz., var. grandis and var. orientale. 10 g of air dried powder was extracted with 60 mL of solvents viz., petroleum ether, chloroform and ethanol by cold extraction. TLC was carried out on 10 × 20 cm silica gel plates (Merck, Germany). TLC phenolics and steroids profile was detected using the specific mobile phase and spraying agents. The appearance of blue colour and bluish green colour spot in the TLC chromatogram indicated the presence of phenolics and steroids in the crude extracts of Blechnum varieties respectively. The results of the present study revealed the phenolics and steroids profile with varied Rf values. The developed TLC will help the manufacturer for quality control and standardization of herbal formulations and also act as a biochemical marker for this medicinally important plant in the pharmaceutical industry.
