Abstract
A new simple, accurate, precise and reproducible RP-HPLC method has been developed for the estimation of Ondonsetran in its bulk & different pharmaceutical dosage forms using inertsil ODS C18 column (250 x 4.6 mm, 5 μm) in isocratic mode. The mobile phase consists of KH2PO4+ACN: MeOH (30:40:30) v\v pH6. The detection was carried out at 216 nm for Ondonsetran. The method was linear over the concentration range48-112 μg/ml for Ondonsetran in tablet and the recovery of Ondonsetran in tablet was found to be 100.91respectively. The retention time was found to be 3.4. The % RSD for precision & accuracy was found to be less than 2%.The validation of method was carried out utilizing ICH-guidelines. The described HPLC method was successfully employed for the analysis of pharmaceutical formulations containing Ondonsetran in different dosage forms i.e. in tablet.
