Abstract
Oxidative-nitrosative stress and advanced glycation end products (AGEs) formation plays an important role as an early etiologic factors of diabetic complication and later progression. Presently no preventive treatment is available for diabetic complications. In the present study we have evaluated and compared the preventive effect of nebivolol (NEB), a β1-receptor antagonist with vasodilator property and l-methyl folate(LMF), an active form of folic acid in the progression of diabetic complications i.e. nephropathy and neuropathy. In vitro studies were performed using antioxidant activity, nitric oxide scavenging activity and AGEs inhibitory. Further in vivo studies were also performed by administrating NEB (1mg/kg & 2mg/kg p.o./day) and LMF (5mg/kg & 10mg/kg p.o./day) to the diabetic rats for 8 weeks. Our result shows that NEB and LMF significantly ameliorate alterations in serum albumin, serum creatinine and plasmatic nitric oxide levels. Interestingly LMF have shown good in vitro AGEs inhibitory activity as compared to NEB. Result of the present study suggest the protective effects of NEB and LMF in the progression of diabetic complications. Therefore NEB and LMF can be administered in combination with antihyperglycemics for the management and treatment of diabetic complications.
