Abstract
In vitro explants response and regeneration capacity of Glycyrrhiza glabra L. was tested by using varying concentrations of BA, Kn, NAA, and IBA on MS media. Explant types exhibited a different response towards establishment and shoot proliferation with highest shoot number at different concentration of PGR’s. By contrast, the nodal expant has led to the highest percent establishment and remarkable enhancement in shoot proliferation. The percent explants establishment (66.67%) and shoot formation frequency was highest (3.67 shoots/explants) at the concentration of BA 2.0 mg/l. A good response was observed towards multiplication of shoot highest in 2.0 mg/l BA + 0.50 mg/l NAA which lead to the best rate of regeneration frequency (86.67%) and shoot formation (18.33 shoots/explants), whereas 1.0 mg/l BA + 0.25 mg/l NAA favored the highest shoot development (24.0 shoots/explants) with apical buds after 4th weeks of inoculation. The study on rooting capacity revealed that treatments 1.0 mg/l IAA proved to be superior with maximum 100% rooting, 16.0 roots/explant and 2.33 cm root length. Present investigation reports that the response of nodal explants found to be the best for explants establishment and hormonal concentrations respectively for regeneration of medicinally important plant of Glycyrrhiza glabra L.
