Abstract
The present work describes development and validation by high-performance liquid chromatography–diode array detection (HPLC–DAD) procedure for the analysis of Montelukast and Bambuterol in pharmaceutical mixture. Effective chromatographic separation of Montelukast and Bambuterol was achieved using a C8 AGILIENT (4.6 mm, 250 mm, and 5 μm) column with isocratic elution by the mobile phase composed of 0.1% solution of orthophosphoric acid (OPA) in water and methanol in perportion of (10:90) respectively. The flow rate is 0.7ml/mint on detecting wavelength 225nm. The proposed HPLC method was statistically validated with respect to linearity, ranges, precision, accuracy, selectivity, LOD, LOQ and robustness. The retention time (RT) of Bambuterol and Montelukast and were found to be 3.500 and 6.4167 respectively. All parameters were found to be within the acceptance limit. The calibration curves were linear in ranges of 10-50, μg/ml for respectively. The r2 of Bambuterol and Montelukast was found to be 0.999, 0.999 respectively.
