Abstract
Objective: The purpose of the present study is to investigate possible toxic reproductive effect of TC in Wistar rats. To evaluate the toxicity profile of TC, either before mating or during pre and post embryo implantation periods upto one generation. Method and Materials: All the individual herbs were mixed and the extract was standardized through HPLC to confirm the desired constituent. The reproductive toxicity of TC extract was evaluated on Wistar rats on both sexes. Animals were divided into four groups, one control group as group-1 which was untreated and three test groups as group-2 (250 mg/kg extract), group-3 (500 mg/kg extract) and group-4 (1000 mg/kg extract). All the animals were treated orally. Twenty rats per sex per dose group were selected for the study. Toxicity of TC extract was evaluated on parent generation (F0) and their progeny (F1) based on different parameters as implantation, estrous cycle, hematology, biochemical analysis, organ necropsy, urine analysis etc. Results: The results showed that exposure of the F1 progeny during the pre and post-mating periods did not significantly alter the reproductive capacity. However, in higher doses reduction of fetal weight was observed. All the treated groups were devoid of any sign of toxicity. Conclusion: At the end of the reproductive toxicity study in rats it can be concluded that no signs of abnormalities observed in any of the vital organs in both parent and F1 generations. Hence, TC is safe for long term use.
