Abstract

The present work describes a simple, selective, rapid, accurate and economic method which has been developed and validated for simultaneous estimation of Escitalopram oxalate (ESC) and Etizolam (ETZ) in tablet dosage form by reverse phase Ultra Performance Liquid Chromatography (RP-UPLC) with PDA detector. The chromatographic separation was carried out on a column Symmetry C18 (4.6×50mm, 5m) by using mobile phase mixture of buffer (pH was adjusted at3 by using ortho phosphoric acid) and acetonitrile at ratio of 85:15 v/v at a flow rate 0.5 ml/min. The PDA detection made at 230 nm. The retention time is for Escitalopram oxalate and Etizolam was found 0.752 ±0.002 min & 2.612 ±0.003 min respectively with a run time of 4 minute and resolution of 22.41. Calibration plots were linear over the concentration ranges 80-120 μg/ml and 8-12 μg/ml for ESC and ETZ respectively. The developed method was validated in terms of linearity, accuracy, precision, limit of detection, limit of quantitation and robustness as per ICH guidelines given for analytical method validation and the method was also found to be simple, sensitive, accurate and precise.