Abstract
The aim of this study was to validate a Reversed-phase High Performance Liquid Chromatographic (RP-HPLC) method for the quantitative determination of hyperoside contained in a cream preparation. The samples were extracted by using ethanol based ultrasound-assisted extraction. The chromatography column used was 5 μm Zorbax Extend RP-18 column (4.6 mm × 250 mm). Mobile phase was 2 % v/v acetic acid and acetonitrile and a gradient system was used. The flow rate was 1.0 ml/min and the detective wavelength was 340 nm. Injection value for each time was 20 μl and separations were carried out isothermally at 30 °C in a heated chamber. The calibration curve was linear (r= 0.999) at the range of 2-10 mg/100 ml. The relative standard deviation of the intraday and interday test results were between 1.55-2.35 % and 0.25-1.72 % respectively. The limit of detection and quantification were 0.06 mg/mL and 0.18 mg/mL. The mean recovery rate was 91.85-103.23 %. The average content of hyperoside in its preparation of three batches was 0.27±0.01 mg in 12 g of the cream. These results clearly showed that the method was rapid, simple, specific, precise and accurate.
