Abstract

A simple high-performance liquid chromatographic method was developed and validated for the determination of lopinavir in rat plasma using nevirapine as internal standard. The separation was achieved on Agilent C8, 150mm* 4.6, 5μ column with isocratic flow. The mobile phase at a flow rate of 1.5ml consisted of 0.05M Potassium Dihydrogen Orthophosphate buffer and Acetonitrile:MeOH in the ratio of (80:20).The ratio of buffer:organic was (45:55).The UV detection was carried out at 210nm. The method was successfully validated in accordance to ICH guidelines. This method was then used to study the pharmacokinetics of the drug when administered orally. The study thus provides a simple precise robust bio-analytical method with its further implications in pharmacokinetics.