Abstract

Plant tissue culture is a potentially useful technique for growing endangered or threatened plants, sometimes it’s difficult to discover the favorable conditions for re-establishment and sustainable growth of plants. The present study was carried out to study the in vitro callus, shoot and root induction from different explants (leaf and petiole) from medicinally important plant Orthosiphon stamineus Benth. Significantly more shoots were induced on the medium containing IBA (1.5 mg/l) than any other treatment. Sub-culturing regenerated shoots on a medium with (IBA 1.5 mg/l) induced the maximum rate of shoot multiplication. Methanol extract potentially decomposed the free radicals by in vitro antioxidant of DPPH at low concentration (30 μg/mL). In the present study, O. stamineus possessed the highest antioxidant and antibacterial activities and thus could be a potentially rich source of natural antioxidants.