Abstract

A simple, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed and validated for the estimation of Irbesartan. AMicrosorb-MV 100-5 C-18 (250 x 4.6mm, 5 μm) column using UV detector with mobile phase containing methanol, 0.02% Formic acid (70:30, v/v) was used. The flow rate was 1.0 ml/min and detection was carried out at 234 nm. The retention time for Irbesartan was 8.18 min. The method was validated for linearity, accuracy, precision, limit of detection, limit of quantification and robustness. The results of all the validation parameters were well within their acceptance values.The proposed method was successfully applied for the quantitative determination of Irbesartan.