Abstract
A reverse phase high performance liquid chromatographic method was developed for the determination of Mizolastine in bulk and in Pharmaceutical dosage form. The separation was effected on a Hypersil BDS C18 column (150 mm x 4.6 mm; 5μ) using a mobile phase composition was 1.36 gms mobile phase mixture buffer of potassium dihydrogen phosphate and acetonitrile (40:60 v/v) and the PH was adjusted to 6.5 with 1N Potassium Hydroxide at a flow rate of 1mL/min. The detection was made at 287 nm. Calibration curve was linear over the concentration range of 5μg/mL – 30μg/mL of Mizolastine. The propose method was validated as per the ICH guidelines. The method was accurate, precise, specific and rapid found to be suitable for the quantitative analysis of the drug and Pharmaceutical dosage form.
