Abstract
The main object of this work was to develop and validate a simple, precise, fast, and accurate RP-HPLC method for the determination of Zidovudine and Nevirapine, an anti-viral in bulk dosage forms .Zidovudine acts as a chain terminator of viral DNA during reverse transcription. Nevirapine belongs to the dipyridodiazepine chemical class. Chromatography was carried out on an Symmetry C18 (250x4.6mm, 5μm) column with a mobile phase of water and methanol (40:60 v/v). The flow rate was 0.8ml/min. The detection wavelength was carried out at 250nm. The retention time is 3.837minutes for Zidovudine and 5.016minutes for Nevirapine. The linearity was found in the range of 0.080 - 0.120 mg/ml (r=0.999) and % RSD is less than 2%. The mean recoveries obtained for Zidovudine were in the range of 99.9 – 100.3% and Nevirapine were in the range of 100.0 -100.5%. The method was successfully validated for specificity, linearity, precision, accuracy, robustness, limit of detection & limit of quantification and solution stability. This method can be successfully employed for the simultaneous determination of Zidovudine and Nevirapine in Bulk forms.
