Abstract

The proteins from aqueous extract of Bacopa monnieri plant leaves were studied to evaluate its antioxidant activity by in vitro methods. The phytochemical analysis and antioxidant activity of Bacopa monnieri plant leaves proteins was evaluated. The quantitative phytochemical study was done for the dialyzed ammonium suphate precipitated proteins. The crude extract had more sugars (150.63mg/g), proteins (10.54mg/g) and the dialyzed protein precipitate contains more proteins (8.82mg/g) and negligible amount of other phytochemicals. Free radical scavenging activity was evaluated using 1, 1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and nitric oxide scavenging assay. The study reveals that, the Bacopa monnieri plant leaves proteins showed potent antioxidant activity in comparison with standard antioxidants like Ascorbic acid and BHA at a lower dose of 10μg. The above result shows that, the proteins from Bacopa monnieri plant leaves has significant antioxidant activity.