Abstract
To develop a simple, precise, accurate, and stability indicating a UV-method for estimation of rilpivirine hydrochloride. Method A involves the method development and solvent was used as a methanol and water (8:2). Linearity in the concentration range of 0.5–3.5 μg/ml and absorbance was measured at 305 nm. Method B involves forced degradation studies of rilpivirine hydrochloride were carried out under hydrolytic, oxidation and photolytic as per stability indicating assay methods at different time intervals 0.5, 1, 2, 3,4,6, 12 and 24 hr and different temperature like room temp, 600 and 90.0 The low % RSD values are indicates the accuracy and precise of the method. The proposed methods can be successfully applied for method development, validation and stability study of rilpivirine hydrochloride. Degradation of rilpivirine hydrochloride was found to occur in alkaline, oxidation and neutral conditions whereas it was found to be stable in acidic and photolytic condition. The kinetic parameters (rate constant, t1/2, and t90) of the degradation of rilpivirine hydrochloride were calculated.
