Abstract

In the Arthritis, immune system of the body will target its own tissues and results in Inflammation. Furthermore, Signalling Pathway like Akt phosphorylation, PI3 kinase activation and jak-stat pathway also plays a major role. This study was carried out to Investigate Anti-Arthritic activity of Psoralea Corylifolea and Clozapine in rats. Psoralidin which is one of constituent of Psoralea Corylifolea inhibits above mentioned Signalling pathway which involves in arthritis. [1] Clozapine shows immunosuppressant effect which proved beneficial role in Rheumatoid Arthritis. In this study, Carrageenan Induced Paw Edema and Complete Freund’s adjuvant (CFA) Induced Arthritis Methods were used to evaluate anti-Inflammatory and anti-arthritic effect of study drugs respectively. In both the methods, selected rats were randomly grouped as per their body weight into normal control, arthritic rats and treated rats (Includes Standard and Test Treatment). The effects of drugs were evaluated by change in paw volume, body weight and arthritis index, change in erythrocyte sedimentation rate (ESR), detection of serum rheumatoid factor (RF), blood cell count, SGOT, SGPT and by histopathology of knee joint before and after treatment. For both the drugs, results showed that % Inhibition in Paw volume was observed 84.53 in Psoralea corylifolea (P) and 60.89 in clozapine(C). Arthritic Index was decrease up to 0.033 in both the test groups. RA factor was not significantly decreased in P extract (50 mg/kg) treated group as compared with Disease control group. But it was significantly decreased in P extract 50 mg/kg+ C 1.25 mg/kg, Methotrexate (1 mg/kg) treated groups. Hb increased 2.25 gm/dl in P, 2.30gm/dl in C. RBCs were significantly increase by 1.36cmm in P, 0.83cmm in C. WBCs were significantly decreased by 0.44 cmm in P while 1.413 cmm in C. SGPT was decreased by 7.08 U/L in P while increased by 13.16 U/L in C. SGOT was decreased by 35.28 U/L in P while increased by 16.88 U/L in C. Combined treatment of P and C (P+C) showed Synergistic effect.