Abstract
In the present study, a simple, selective, precise and accurate HPLC method was developed and validated for the simultaneous estimation of atorvastatin and fenofibrate in bulk and its formulations. The mobile phase consists of a mixture of acetonitrile and water (0.05% OPA) in the proportion 75:25. The HPLC analysis of atorvastatin and fenofibrate was carried out at a wavelength of 248nm with a flow rate of 1ml/min. The linear regression analysis data for the calibration curve showed a good linear relationship with a regression coefficient 0.997 and 0.998 respectively. The linear regression equation was y = 45.06x + 4.177 and y = 28.06x + 213.1. The developed method was employed with a high degree of precision and accuracy for the analysis of atorvastatin and fenofibrate. The method was validated for precision (% RSD 1.50 & 0.14 resp.), accuracy (99.97%) ruggedness, (% RSD 0.28 and 0.48 resp.) robustness, repeatability (% RSD 1.93), LOD(%RSD 0.14 and 0.39 resp.) and LOQ (%RSD 0.48 and 1.19 resp.). From the results we can conclude that this method is better for the estimation of atorvastatin and fenofibrate in bulk and its tablet formulation.
