Abstract
Bioanalytical method is necessitates to understand the pharmacokinetics of any drug and its metabolites. Since plasma is one of most widely adopted biological fluid in drug discovery and development. This article discusses the various bioanalytical methods used for the quantization of drugs and their metabolites in biological fluids. In today’s drug formulation and development environment, highly sensitive and selective methods are required to quantify drugs in biological fluids such as blood, plasma, serum, or urine. Chromatographic methods (high-performance liquid chromatography [HPLC] or gas chromatography [GC] have been widely used for the bio-analysis of small molecules, with liquid chromatography coupled to mass spectrometry (LC/MS),electrochemical ,electrophoretic, titrimetric methods, spectrophotometric techniques etc. After developing a method with desired attributes, the method is validated to establish that it will continue to provide accurate, precise, and reproducible data during study-sample analysis.
