Abstract
To develop and validate a new method for simultaneous estimation of montelukast and levocetirizine in liquid dosage form. Chromatographic separation was achieved isocratically on a Waters C18 column hypersil (4.6mm × 250mm ×5µm) using a mobile phase, 55:45 (phosphate buffer: acetonitrile). The flow rate was 1 ml/min and effluent was detected at 228 nm and 20μl of sample was injected. The run time was found to be 6min. The Retention time of Montelukast and levocitirizine was found to be 4.247 min. and 2.823 min respectively. Linearity was observed in the concentration range of 25-75 % for levocetirizine and 40-120% for montelukast. Percent recoveries obtained for both the drugs were 999-999%. The percentage RSD for precision and accuracy of the method was found to be less than 2% as per the ICH these method was rugged. The percentage purity thus found is 98.65% and 99% for montelukast and levocitirizine. Conclusion:- A simple, selective, linear, precise, and accurate RP-HPLC method was developed and validated for the simultaneous estimation of montelukast and levocetrizine in its bulk and liquid dosage form. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision, robustness and ruggedness. The new rp-hplc method was developed successfully it is applied for the analysis of simultaneous estimation of montelukast and levocetirizine in syrup
