Abstract

The goal of the current investigation was to simultaneously separate the anti-hypertensive agents, amlodipine and metoprolol in bulk and pharmaceutical dosage form. The chromatographic separation for amlodipine and metoprolol was achieved with mobile phase containing acetonitrile and methanol (70:30 % v/v), reverse phase Thermo scientificTM HypersilTM BDS 5μ C18 120A (250 × 4.60 mm i.d ) column in isocratic mode at room temperature and UV detection 222nm. The compounds were eluted at a flow rate of 1.0 ml/min. The retention times of amlodipine and metoprolol were found to be 1.665min and 2.866min respectively. The above method was validated in terms of System suitability, linearity, accuracy, precision, Limit of Detection (LOD), Limit of Quantification (LOQ) in accordance with ICH guidelines. The results which are obtained from the method validation were within the acceptable limit. From the results it was concluded that the method has been developed for the simultaneous estimation of amlodipine and metoprolol in combined dosage form. The developed method was simple, rapid, precise, economical and suitable for routine quality control analysis and quantification of these drugs in combined dosage form.