Abstract

Objective: Several microRNA, which are ~22-nucleotide noncoding RNAs, exhibit tissue-specific or developmental stage–specific expression patterns and are associated with human diseases. The main objective of this study is to identifying expressions profile of microRNA in arthritis to spot novel biomarker. Methods: The expression of miRNA 146 and 181 was recorded by taking the blood samples from 5 with ankylosing spondylitis, 1 with osteoarthritis, 1 with gout, 2 with RA and 5 with other types of arthritis. RNA isolation is done with GITC method followed with the preparation of cDNA, the for the DNA amplification purpose it had been processed further with PCR, then RT-PCR is done in order to obtain values for statistical analysis. Results: Differential expression profile was observed. MiRNA-146 level was showing lower expression levels in all patients and MiRNA-181 was showing higher expression level, however biochemical parameters are not correlated with both the miRNAs. Conclusion: By the above finding it reveals that miRNA-146 could be set as biomarker in identification of arthritis where it is expressing pattern is low when compared with miRNA-181 which is highly up-regulated during inflammatory response. So, both miRNAs can be used as biomarker for diagnosis purpose in correlation with other biochemical parameter.