Abstract
A new simple, accurate, precise and reproducible RP-HPLC method has been developed for the simultaneous estimation of ibuprofen and famotidine and paracetamol in tablet dosage forms using Kromosil ODS column,C18(250x4.6 ID) 5μm in isocratic mode. The mobile phase consisted of Solution used – Phosphate buffer: CAN (40+60) pH 4.0. The flow rate was 1.0 ml/min and detection wavelength was carried out at 230nm. The retention times of ibuprofen and famotidine & paracetamol were 6.477 min and 8.217 min & 2.703 min, respectively. The method was linear over the concentration range for ibuprofen 2-10 μg/ml and for and famotidine 2-10 μg/ml & for paracetamol 2-10 μg/ml. The recoveries of ibuprofen and famotidine were found to be in the range of 99.2% and 99.85% & 100.49% respectively. The validation of method was carried out utilizing ICHguidelines. The described HPLC method was successfully employed for the analysis of pharmaceutical formulations containing combined dosage form.
