Abstract
In the present research work, a simple, accurate, precise, reproducible and cost effective first derivative UV Spectrophotometric method developed and validated for the simultaneous estimation of Sildenafil Citrate and Aspirin in bulk as well as tablet dosage form. The solution of both the drugs and tablet were prepared in Methanol. First derivative quantitative determination of these drugs was performed at 255 nm for Sildenafil Citrate at zero crossing point (ZCP) of Aspirin and at 291nm for Aspirin at zero crossing point (ZCP) of Sildenafil citrate. This method obeys Beer-Lambert’s law in concentration range of 5-30 μg/mL and 10-80 μg/mL for Sildenafil Citrate and Aspirin respectively. Co-efficient of correlation were found to be 0.998 for both the drugs. The % RSD were not more than 2.0 % which indicates good intermediate precision. The values LOD and LOQ were 0.588μg/mL and 1.782μg/ml for Sildenafil Citrate and 1.08μg/ml and 3.27μg/ml for Aspirin respectively. Percentage estimation of Sildenafil Citrate and Aspirin in tablet dosage form were found to be 101.00 % and 99.47% respectively.
