Abstract
Two simple, precise, rapid and accurate methods for simultaneous determination of lamivudine (LAM), tenofovir (TEN) and nevirapine (NEV) in combined tablet dosage form are developed. Method A is based on ratio spectra derivative and method B is based on derivative spectra, methanol is used as solvent for both these method. Quantitation by ratio derivative amplitude was achieved at 352.9 nm, 359.9 nm and 348.9 nm for lamivudine, nevirapine and tenofovir, respectively for method A. Similarly the amplitudes at 324.01nm, 298.9 nm, 311.9 nm were measured for lamivudine, tenofovir and nevirapine for receptive drugs in method B. Beer’s law is obeyed in the range of 9-27 μg/ml by lamivudine, tenofovir and 6-18 μg/ml by nevirapine. The percentage assay in laboratory formulation was found to be in the range 99.39%100.23% by the proposed methods. The methods were validated with respect to precision, accuracy, specificity and robustness. The recovery was found in the range of 99.21-101.13 %. The methods developed are simple, economical, precise and accurate and can be used for routine quality control of analytes in combined tablets.
