Abstract

A simple and selective RP-HPLC method is described for the determination of Acetaminophen and Tramadol dosage forms. Chromatographic separation was achieved on a C18 column using mobile phase consisting of a mixture of mixed Phosphate buffer pH: 3.4 Acetonitrile (30:70v/v/v), with detection of 236nm and flow rate at 1.2mL/min. Linearity was observed in the range 100-300 μg /ml for Acetaminophen (r2 =0.99) & 10-30μg /ml for Tramadol (r2 =0.99) for the amount of drugs estimated by the proposed methods was in good agreement with the label claim. The proposed methods were validated. The accuracy of the methods was assessed by recovery studies at three different levels. Recovery experiments indicated the absence of interference from commonly encountered pharmaceutical additives. The method was found to be precise as indicated by the repeatability analysis, showing %RSD less than 2. All statistical data proves validity of the methods, sensitivity, precision and reproducibility. It can be used for routine analysis of pharmaceutical dosage form.