Abstract
Diabetes mellitus is characterised by hyperglycaemia and unbalanced carbohydrate metabolism. Under hyperglycaemic condition, regulation of blood glucose level happens to be indispensable for preventing various diabetes related complications. Treatment with herbal drugs has always remained a choice of treatment for protecting or rejuvenating β-cells and smoothing out fluctuation in glucose levels. A polyherbal medicine (PHM) formulated and tested with different in-vitro anti-diabetic assays in present study indicate that PHM possesses good antidiabetic activity. In vitro human salivary amylase (HSA) inhibition assay exhibited 81.27 % amylase inhibition and yeast glucose uptake assay showed 25% increase in glucose uptake at the concentration of 250 mg ml-1. Generally the haemoglobin glycosylation is determined mainly to identify the average glucose concentration over a prolonged period of time. Higher values of glycated haemoglobin indicate poor control of blood glucose level under diabetic condition. The in-vitro haemoglobin glycosylation inhibition assay showed considerable inhibition of glycosylation over a period of 72 hrs as compared to gallic acid, which clearly indicates the capacity of PHM to decrease the formation of glucose-haemoglobin complexes at physiological glucose concentration. Overall results of in-vitro antidiabetic assays clearly indicate that PHM has considerable antidiabetic activity and may be used in management of hyperglycaemia in diabetic patients.
