Abstract
Present work describes a precise, accurate and reproducible reverse phase high performance Liquid Chromatographic (RP-HPLC) methods for simultaneous estimation of Lornoxicam (LXM) and Eperisone (EPE). Both the drugs were resolved in a synthetic mixture, using a 250 mm x 4.6 mm, 5 μm particle size, C18H column using acetonitrile:water (70:30 v/v) mixture as a mobile phase of pH 2.8 adjusted with triethanolamine and orthophosphoric acid. The detection of the synthetic mixture was carried out at 291 nm with a flow rate of 0.8 mLmin-1. The retention times were 3.350 and 4.710 minutes for EPE and LXM, respectively. The correlation coefficient was 0.9975 and 0.9987 for EPE and LXM, respectively. The relative standard deviation values for repeatability and intermediate precision studies were less than 2 %, and % recovery was greater than 98% for both drugs. The developed method was found to be suitable for the routine estimation of EPE and LXM in tablet dosage form. As per ICH guidelines the developed method were validated in terms of linearity, precision, accuracy, limit of detection and limit of quantification, and the results were found to be satisfactory.
