Abstract
The present study was aimed to reveal the alkaloid profile of Clitoria ternatea seed, stem and leaves using HPTLC. Preliminary phytochemical screening was carried out by Harborne method. HPTLC studies were followed by Harborne and Wagner et al. method. The ethyl acetate-methanol-water (100: 13.5: 10) was employed as mobile phase for alkaloids. The methanolic extract of stem, leaves and seeds of Clitoria ternatea showed the presence of 26 different types of alkaloids with 21 different Rf values with range 0.02 to 0.93. In general more degree of alkaloids diversity has been observed in reproductive parts (seeds) when compared to the vegetive parts leaves and stem. Maximum number (10) of alkaloids has been observed in seeds followed by leaves (9). Among the ten different alkaloids of seeds, seven (0.15, 0.23, 0.41, 0.52, 0.62, 0.67 and 0.79) are unique to the seeds only and they are not present in the vegetaive parts of the plant. The proposed HPTLC profile can be used for the identification of the medicinally important plants and distinguish from its adulterant.
