Abstract
An isocratic Simultaneous estimation by RP-HPLC Method were developed and validated for the Estimation of Lamivudine, Stavudine and Efavirenz in Bulk and tablet dosage form. Quantification was achieved by using a reversed-phase L10 column (ZORBAX SB CN Column, 5μ, 150 mm × 4.6 mm) at ambient temperature with mobile phase consisting of 0.2% Triethylamine: Acetonitrile (40:60 pH: 2.0)). The flow rate was 0.6 mL/min. Measurements were made at a wavelength of 258nm. The average retention time for Lamivudine, Stavudine and Efavirenz were found to be 2.4 min 2.8 and 5.6. The proposed method was validated for selectivity, precision, linearity and accuracy. The developed method was successfully applied to estimate the amount of Lamivudine, Stavudine and Efavirenz in bulk and tablet dosage form.
