Abstract
The effect of NLE was studied on separate set ulcer model, which enabled detailed observation of the antisecretory and antiulcer effect of NLE. NLE in dose of 20,40, 80 and 160 mg/kg was administered by subcutaneously route immediately after pyloric-ligation. The advantage of this mehod is that it allows analysis of the gastric contents (volume, pH, pepsin, mucous, polysaccharides etc) and study of the stomach mucosa as well for the occurrence of ulceration, petechiae, hemorrhage etc. Anatomically, the Albino mice stomach is divided into upper two-fifth non-secretory portion and lower three-fifth glandular secretory portion, which resembles the body of the stomach in man both anatomically and functionally. NLE within the dose range of 20 to 160 mg/kg reduced the mean volume in a dose- dependent manner. The range gastric secretion in each group gradually decreased with concomitant increase in dose. A 160 mg/kg dose NLE produced a volume reduction to fifty percent of the control value. The volume of gastric secretion was significantly reduced by ranitidine 25 mg/kg. This experiment has clearly brought in to focus the effect of NLE in reducing gastric volume of the gastric acids content. The optimum result has been obtained in the dose of 80mg/kg and 160mg/kg of body weight. Following the administration of NLE in doses of 20, 40, 80 and 160 mg/kg, the value of mean number of ulcers per albino mice stomach recorded in this investigation were 4.67, 4.3, 3.1 respectively. Significant reduction was obtained by dose of 80 mg/kg and 160 mg/kg in our study.
