Abstract

Before fitting with a receptor a drug has to cross several bio-membranes, containing lipid as one of the important components. Interaction of a drug with lipid may lead to lipid peroxidation, an oxidative process which produces several toxic end products, including malonyldialdehyde (MDA), 4-hydroxy-2-nonenal (4-HNE) etc. Antioxidants have the capacity to minimize lipid peroxidation. In view of the above context the present in vivo study was conducted to find out lipid peroxidation induction potential of Tobramycin, an aminoglycoside antibiotic and its inhibition with Ascorbic acid, an antioxidant. Rabbits are used as animal model. Rabbits divided into different experimental groups are treated with drug and co-administered with ascorbic acid. The levels of MDA, HNE, GSH and NO in the blood are estimated and compared with the control. The results revealed that Tobramycin significantly increases MDA and HNE contents, but decreases GSH and NO levels; and though ascorbic acid shows pro-oxidant effect but on co-administration with tobramycin, ascorbic acid has the capability to inhibit drug-induced lipid peroxidation.