Abstract

In the present investigation was aimed to isolate and identification of bioactive compound from Crotalaria pallida endophytic fungal Alternaria species ethanol extract and evaluated for in vitro cytotoxicity assays using onion roots, yeast and greengram germination method. MAE method was used for isolation of potent compound and subjected to purification from HPLC with standard compounds. In vitro cytotoxicity methods were used to know the toxicity level of the isolated compound. MAE method yielded major bioactive compounds along with three coumarin(s) and it was confirmed with all four tests and the 5th test confirmed as a phenolic agent. Endophytic fungal coumarin inhibited the actively growing onion root meristematic cells by possessing various abnormalities by producing highest percentage of cytotoxicity (18.05%), the death of yeast was observed in extract treated yeasts and the percentage of toxicity was 88.03% and at 400 μL of endophytic coumarin inhibited the greengram germination and there is no germination was observed. The fungal coumarin also cleaved the DNA in yeast and it is a strong evidence of toxicity in DNA level and shown apoptosis activity. For all experiments, standard coumarin was used. The endophytic fungal coumarin results were near to equal of standard. From the obtained results, the endophytic fungi Alternaria species have the ability to produce coumarin and it exhibited as strong in vitro cytotoxicity activity. It can be used as a cancer drug after test against cancer cell lines.