Abstract
A simple, selective, linear, precise, and accurate RP-HPLC method was developed and validated for the simultaneous estimation of Metformin and Telmisartan from bulk and formulations. Chromatographic separation was achieved isocratically on a Waters C18 column (250×4.6 mm, 5 μ particle size) using a mobile phase, (Mixed Phosphate buffer pH adjusted to 3.0 with orthophosphoric acid& acetonitrile in the ratio of 50:50 v/v. The flow rate was 1 ml/min and effluent was detected at 234 nm and 10μl of sample was injected. The retention time of Metformin and Telmisartan were 2.257 and 5.790 min respectively. Linearity was observed in the concentration range of 30-90 μg/ml for Metformin and 2.4-7.2 μg/ml for Telmisartan. Percent recoveries obtained for both the drugs were 98-101%and99-100 respectively. The percentage RSD for precision and accuracy of the method was found to be less than 2%. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision, LOD and LOQ. The method developed was successfully applied for the analysis of simultaneous estimation of Metformin and Telmisartan tablets.
