Abstract
The present work includes a simple, inexpensive, rapid, accurate and precise UV spectrophotometric method and isocratic RP-HPLC method for estimation of Edaravone in bulk and its formulation. Estimation was done at 243nm which was the λmax of Edaravone. The simple, isocratic RP-HPLC method involves separation of Edaravone on Reverse phase C18 Kromasil column (250 mm x 4.6 mm, 5 μm ) with a Mobile phase of Water: Acetonitrile = 55:45. The developed methods were validated successfully according to ICH Q2 (R1) guidelines. Both methods showed a good linear response with r2 values of 0.999 and 0.9994 respectively. The percentage Relative Standard Deviation for both methods was found to be less than two, indicating that the methods were precise. The mean percentage recovery for UV and RP-HPLC method was between 99-102% and 99-101% respectively. Edaravone in its injection formulation could be accurately determined with assay values ranging from 100- 101.5 %. Both the developed methods were specific, selective and robust. The methods could be successfully applied for analysis of injection formulation of Edaravone.
