Abstract

The present study was conducted to evaluate the hepatoprotective activity of phytoconstituents of methanolic extract of leaves of Wrightia tinctoria against D-galactosamine induced liver damage in albino rats. The phytoconstituents (100 mg/kg) was administered orally to the animals with hepatotoxicity induced D-galactosamine (400 mg/kg for two days). Silymarin (50 mg/kg) was given as standard hepatoprotective drug. All the test drugs were administered orally in the suspension form to the animals. The combination of silymarin and methanolic extract of Wrightia tinctoria leaves were also given to animals to compare with individual groups as well as toxicant group. Combination of silymarin and methanolic extract of Wrightia tinctoria leaves were effective in protecting the liver against the injury induced by D-galactosamine in rats. This was evident from significant reduction in serum enzyme alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and total bilirubin. These biochemical observations were also supplemented by Histopathological observations of livers sections. It was concluded from the results that the combination of silymarin and methanolic extract of Wrightia tinctoria leaves reduces the hepatoprotective activity against D-galactosamine induced hepatotoxicity in rats.