Abstract
Two spectrophotometric methods Zero crossing derivative and ratio- spectra derivative spectrophotometric methods were developed for the determination of Mometasone furoate (MF) and Formoterol fumarate (FF) in the pharmaceutical dosage form. Zero crossing dervative spectrophotometry involves amplitudes measurement of the first derivative spectra of the standard and sample solution at 267.56 nm for MF and 306 nm for FF. Ratio spectra derivative spectrophotometry involves amplitudes measurement of the ratio first derivative spectra at 263nm and 267.30 nm for MF and 293.08 and 302.60 nm for FF. The calibration graph follows beer’s law in the range of 30-200 µg /ml of MF and 2-6 µg /ml for FF. The accuracy and precision of the method were validated statistically. So it can be a preferable method for routine analysis due to its simplicity and economical advantages.
